A single mutation on the amino acid residue may cause a severe change in the whole protein structure and thus, lead to disruption of function. Predicting the protein stability changes can provide several possible candidates for the novel protein designing. We first proposed an integrated predictor, iStable, to solve the conflicting prediction results from different tools could cause confusion to users (1). In this web server issue, a new web tool, iStable 2.0, was constructed for three purposes. 1) To construct stand-alone program module for reducing the risk of broken of sub prediction web servers. 2) To add in sequence coding and XGBoost for improving the prediction accuracy. 3) To visualize four kinds of output data for protein designing. The flowchart of iStable 2.0 is as follows:

System Architecture


Chi-Wei Chen, Jerome Lin, Yen-Wei Chu (2013) iStable: Off-the-shelf Predictor Integration for Predicting Protein Stability Changes, BMC Bioinformatics, 14(suppl 2):S5, doi:10.1186/1471-2105-14-S2-S5 [PMID: 23369171]